Analytical performance of free and total thyroxine assays
نویسندگان
چکیده
منابع مشابه
Analytical performance of free and total thyroxine assays.
Excessive bias and imprecision are major analytical problems associated with some assays for free and total thyroxine (T4). Bias in free T4 methods is largely proportional to variations in serum T4 binding. In direct methods, this is attributable to requirements for substantial quantities of protein-bound T4 to replace analytical losses of free T4. In some total T4 methods, bias is inversely pr...
متن کاملEvaluation of Total Antioxidant, Total Calcium, Selenium, Insulin, Free Triiodothyronine and Free Thyroxine Levels in Cows with Ketosis
Ketosis is an important metabolic disease of high milk-producing cows. There are significant changes in many metabolite and hormonal concentrations in metabolic diseases. This study was carried out to assess the concentrations calcium (Ca), selenium (Se), total antioxidant (TAOC), insulin, free triiodothyronine (fT3) and free thyroxine (fT4) in cows with subclinical and clinical ketosis. This s...
متن کاملFurosemide interference in newer free thyroxine assays.
To the Editor: The Technical Brief by Hawkins (1 ) refers to the increase in measured serum free thyroxine (FT4) concentrations by furosemide as “interference”, and this deserves comment. As discussed previously (2, 3), one can legitimately conclude that an assay is unaffected after the addition of exogenous substances (such as thyroid hormone-binding proteins or binding inhibitors) if the assa...
متن کاملtotal thyroxine, free TBG capacity, and free thyroxine index using Sephadex G-25 and '25I-labelled triiodothyronine and thyroxine
SYNOPSIS A trial was carried out on 134 patients of new kits (Ames Co) using columns of Sephadex G-25 for the determination of serum total thyroxine (Tetralute test) and for the indirect estimation of serum free thyroxine-binding globulin capacity (Trilute test). Both new methods were quicker and easier than the reference resin methods and of similar precision. The two measurements when combine...
متن کاملQuantitative determination of total and free triiodothyronine and thyroxine.
ƒÊg per 100ml plasma, respectively. Mean normal fT3 and fT4, calculated from tT3, tT4 and EF values of T3 and T4, were 2.43 and 2.21mƒÊg per 100ml plasma, respectively. The fT3 values can be obtained by the present method more easily and speedily than by the method of Nauman et al. The present fT4 values, in con sideration of BEI and the percentage of T4 in plasma, represent a more accurate one...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1996
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/42.1.146